{"id":118691,"date":"2013-05-22T17:20:23","date_gmt":"2013-05-22T20:20:23","guid":{"rendered":"http:\/\/revistapesquisa.fapesp.br\/?p=118691"},"modified":"2015-06-08T15:20:25","modified_gmt":"2015-06-08T18:20:25","slug":"address-for-excellence","status":"publish","type":"post","link":"https:\/\/revistapesquisa.fapesp.br\/en\/address-for-excellence\/","title":{"rendered":"Address for excellence"},"content":{"rendered":"
\"LaCTAD

LEO RAMOS<\/span>LaCTAD is equipped\u00a0with an isothermal titration\u00a0calorimeter…LEO RAMOS<\/span><\/p><\/div>\n

The University of Campinas (Unicamp) has set up a laboratory that combines in a single location state-of-the-art equipment designed for research in genomics, bioinformatics, proteomics and cell biology. Located in the university\u2019s Science and Technology Park and patterned along the lines of university research facilities abroad, the Central High Performance Technologies Laboratory (LaCTAD) seeks to ensure a high level of quality in the research conducted at Unicamp and in S\u00e3o Paulo State \u2013\u2013 the facilities are made available to researchers from other institutions. \u201cThe university has signed two significant agreements as a result of the laboratory\u2019s existence. This unit will be very useful for research into the proposed areas and will give a major boost to Brazilian science,\u201d asserted Unicamp\u2019s President, Fernando Ferreira Costa, at the inaugural ceremony.<\/p>\n

FAPESP invested approximately R$5.5 million in the purchase of laboratory equipment under the Multi-user Equipment Program (EMU), while building construction and the hiring of staff were left to the university. \u201cIt is notable that Unicamp has invested almost as much as FAPESP, and that LaCTAD has a well-demonstrated cost structure and institutional support critical to hiring employees in bioinformatics and technical support staff with doctorates,\u201d said Carlos Henrique de Brito Cruz, FAPESP’s scientific director, who attended the opening of the laboratory on March 1. The proposed creation of LaCTAD was submitted as part of the notice of request for proposals of FAPESP\u2019s Multi-user Equipment Program in 2009. In 2011 the offer of services in temporary facilities in teaching and research units was initiated.<\/p>\n

\"...a

leo ramos<\/span>…a chromatograph…leo ramos<\/span><\/p><\/div>\n

Three modern sequencers have been acquired for work in the field of genomics. There are two models. One is the Illumina HiSeq 2500, which enables complex sequencing studies through its ability to produce a large number of genome sequences for bioinformatic analysis. The other model is the ABI 3730XL DNA Analyzer, made by Applied Biosystems, which does not produce as many sequences as the other model, but has the ability to map a larger number of base pairs. \u201cIt\u2019s not easy to find a scientific study in the life sciences\u00a0 published by a leading journal that does not contain some element of gene sequencing or changes in the genome, and doesn\u2019t use such data to design the research or plan the experiments,\u201d says Ronaldo Pilli, Unicamp\u2019s Dean of Research. \u201cThis equipment is improving the quality of research carried out at Unicamp.\u201d Providing bioinformatic services, another LaCTAD purpose, is supported by a bank of computers that includes IBM servers and HP machines. \u201cWe have invested in the training of human resources by offering bioinformatics courses every semester,\u201d says Dean Pilli. About 160 students have already been trained.<\/p>\n

Epilepsy
\n<\/b>One of the ongoing projects at the facility is led by Professor Iscia Lopes-Cendes of the Department of Medical Genetics at Unicamp\u2019s School of Medical Sciences. She is using one of the LaCTAD sequencers in a research project on the molecular mechanisms in the development of epilepsy, which seeks to identify gene expression in the brain tissues of rats. Selected neuronal groups in the hippocampus of animal models that have been induced to present the disease are subjected to deep sequencing in a search for transcripts (messenger RNA) that may be relevant for differentiating the pathological from the normal state. \u201cAs this involves deep sequencing, we needed a fast sequencer and we even helped to upgrade its software with funding from our research project,\u201d she says.<\/p>\n

\"...and

LEO RAMOS<\/span>…and a confocal microscopeLEO RAMOS<\/span><\/p><\/div>\n

Professor Gl\u00e1ucia Mendes de Souza of the University of S\u00e3o Paulo (USP) Chemistry Institute, who conducts research and is one of the coordinators of FAPESP\u2019s Program for Research on Bioenergy (BIOEN), also used the services of the Unicamp facility <\/i>in sequencing a sugarcane reference genome. \u201cLaCTAD is providing sequences obtained by using the Illumina sequencer, which complement the sequences we determined with the Roche 454 sequencer. We have one 454 at USP, but not an Illumina, hence the importance of the services they provide,\u201d says Professor Mendes de Souza. Paulo Arruda of Unicamp\u2019s Institute of Biology has also been using the LaCTAD services. A project of his doctoral student Vagner Katsumi Okura involves constructing and sequencing the bacterial artificial chromosome (BAC) library of sugarcane. BAC libraries are fundamental tools for characterizing chromosomal regions that contain genes of interest. A second research project, by doctoral student Pedro Barreto, is investigating how plants regulate mitochondrial biogenesis. The mitochondrion is an organelle responsible for cell bioenergetics. \u201cThere is a reasonable knowledge base about mitochondrial biogenesis in mammals, but little is known about it in plants,\u201d says Arruda, whose work envisions sequencing plant RNAs that overexpress the mitochondrial uncoupling protein (UCP1).<\/p>\n

In the field of proteomics, LaCTAD has liquid chromatography equipment, which can analyze and purify proteins, as well as a calorimeter, which is used to determine the thermodynamic parameters of biochemical interactions. A mass spectrometer, model Xevo Q-TOF MS, belonging to Unicamp\u2019s Chemistry Institute will be made available to LaCTAD\u2019s users until the laboratory purchases its own equipment. As far as the field of cell biology is concerned, the laboratory is equipped with a Leica confocal microscope capable of producing high resolution fluorescence images of a variety of biological samples of materials. Another piece of equipment is a Bio-Rad multiplex immunoassay instrument, which is capable of quickly and accurately assaying dosages of hormones or cytokines, the molecules involved in emitting the signals between cells during immune responses. \u201cWe are organizing an international workshop on cell biology to be held in May. We\u2019re going to hear from outside experts who are conducting the same type of work in the life sciences in a central laboratory so that we can exchange experiences and improve our services. The idea is to generate greater momentum for LaCTAD in cell biology,\u201d says Dean Pilli.<\/p>\n","protected":false},"excerpt":{"rendered":"Unicamp facility combines modern equipment for research in genomics","protected":false},"author":11,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_exactmetrics_skip_tracking":false,"_exactmetrics_sitenote_active":false,"_exactmetrics_sitenote_note":"","_exactmetrics_sitenote_category":0,"footnotes":""},"categories":[156],"tags":[212,210,237,243],"coauthors":[98],"class_list":["post-118691","post","type-post","status-publish","format-standard","hentry","category-cover","tag-biotechnology","tag-cellular-biology","tag-genetics","tag-innovation"],"acf":[],"_links":{"self":[{"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/posts\/118691","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/comments?post=118691"}],"version-history":[{"count":0,"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/posts\/118691\/revisions"}],"wp:attachment":[{"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/media?parent=118691"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/categories?post=118691"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/tags?post=118691"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/coauthors?post=118691"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}