{"id":235298,"date":"2017-03-27T17:45:04","date_gmt":"2017-03-27T20:45:04","guid":{"rendered":"http:\/\/revistapesquisa.fapesp.br\/en\/?p=235298"},"modified":"2017-03-28T14:00:22","modified_gmt":"2017-03-28T17:00:22","slug":"ready-to-compete","status":"publish","type":"post","link":"https:\/\/revistapesquisa.fapesp.br\/en\/ready-to-compete\/","title":{"rendered":"Ready to compete"},"content":{"rendered":"
\"USP

Eduardo Cesar<\/span><\/a> USP researchers work to synthesize spider silk proteins from microalgaeEduardo Cesar<\/span><\/p><\/div>\n

A group of students at the University of S\u00e3o Paulo (USP) has specialized in organizing teams to take part in international science competitions. Since 2012, the Synthetic Biology Club at USP has prepared undergraduate and graduate students, post-doctoral fellows, and faculty members to compete in the International Genetically Engineered Machine Competition (iGEM), held annually in the United States. Two years ago, a team that also included members from S\u00e3o Paulo State University (Unesp) and the Federal University of S\u00e3o Carlos (UFSCar) won a bronze medal for their project, which proposed a method of diagnosing chronic kidney disease (CKD) using biomarkers. Two other teams that were organized at USP during meetings sponsored by the club will compete at iGEM 2016, scheduled for October 27-31, 2016, in Boston.<\/p>\n

Inaugurated in 2004 at the Massachusetts Institute of Technology (MIT), the competition aims to promote the advancement of research on synthetic biology, a field that designs biological devices such as sensors, equipment, and software to solve problems related to the environment, health, food, and energy. Over 300 teams of students and researchers from institutes of higher education worldwide are expected to attend the Boston event this year. \u201cThe Synthetic Biology Club at USP serves to bring people together to conduct projects, organize teams for competitions, and promote discussions of new ideas involving research into molecular and synthetic biology,\u201d explains Otto Heringer, an undergraduate majoring in chemistry at USP and one of the club coordinators.<\/p>\n

One of the teams that will be going to iGEM 2016 employed panels of medium density fiberboard, acrylic sheets, and silicon molds to hand-build an electrophoresis tank and a microcentrifuge, equipment that permits the separation of molecules and biological samples in laboratory experiments. A study initiated in January 2016 employs these devices in the development of an antimicrobial burn dressing. Through genetic manipulation, marine algae can be made to synthesize spider silk proteins, which are used as a raw material. The team, which comprises researchers from USP, Unesp, and the Federal University of S\u00e3o Paulo (Unifesp), will present its preliminary results at iGEM. \u201cOne of the iGEM categories awards teams that design their own hardware. So in addition to competing with our project, we hope to win a medal for the equipment we\u2019ve built,\u201d says Jo\u00e3o Vitor Dutra Molino, doctoral candidate at the USP School of Pharmaceutical Sciences (FCF-USP) and team member.<\/p>\n

\"left

Eduardo Cesar<\/span><\/a> Left to right: Part of the team that will compete in Boston: Mireia Mitter, Tiago Lubiana, Allan Tanaka, Jo\u00e3o Vitor Dutra Molino, and L\u00edvia CamargoEduardo Cesar<\/span><\/p><\/div>\n

The teams who compete at iGEM do not necessarily need to present final results or prototypes. But it is expected that they will demonstrate their project\u2019s potential and present preliminary findings. \u201cWe\u2019ve started the second phase of our research, which involves expressing the spider silk protein in the microalgae. We\u2019re racing against time to wrap up this phase by the time of the competition,\u201d says Molino, who has taken part in earlier iGEM events.<\/p>\n

The 25-member team, which includes professors, researchers, and undergraduate and graduate students, faces not only scientific hurdles; they have also struggled to find funding for the project and cover the expenses of the members who will travel to the United States. \u201cI\u2019ve contributed to the team by reaching out to companies that can help us by donating money or resources needed for the research, like reagents,\u201d says L\u00edvia Seno Ferreira Camargo, a post-doctoral fellow at FCF-USP and one of the team coordinators. The group\u2019s first challenge was registering their project for the event. \u201cEvent registration at iGEM costs $5,000 per team. We obtained support from the multinational pharmaceutical firm Merck, in Germany, which paid for our registration.\u201d<\/p>\n

The researchers also launched a crowdfunding campaign on the Internet and managed to raise some R$5,000. USP kicked in over R$20,000 to cover expenses for its students. According to Camargo, Brazil\u2019s low rate of participation at iGEM is explained in part by the challenges encountered in arranging adequate funding. Only three Brazilian teams will be competing for a prize in 2016. \u201cThere are a lot of synthetic biology research groups in Brazil that would be qualified to compete at iGEM but they can\u2019t afford to register and participate,\u201d she says.<\/p>\n

\"Electrophoresis

Eduardo Cesar<\/span><\/a> Electrophoresis equipment made by the groupEduardo Cesar<\/span><\/p><\/div>\n

Replicated model<\/strong>
\nUSP has a tradition of attending iGEM thanks to student organization. \u201cOne way of organizing is through clubs, whose Brazilian model was born on the USP campus in the state capital and replicated at its campuses in Lorena and Ribeir\u00e3o Preto, as well as at other universities, like the Federal University of Amazonas [UFAM] and Unesp in Assis,\u201d explains Otto Heringer, who points out that student clubs are common at universities abroad.<\/p>\n

For the first time, undergraduate and graduate students at the USP Engineering School of Lorena (EEL-USP) have organized to take on a project for iGEM. Coordinated by geneticist Fernando Segato, professor at EEL, the team is working to produce the components of petroleum-based diesel fuel called alkanes, using Escherichia coli <\/em>bacteria that have been genetically modified to resist fatty acids. This would enable production of oxygen-free oil that would enhance engine efficiency.<\/p>\n

Another Brazilian project that will be presented at iGEM is now under development at UFAM in partnership with Amazonas State University (UEA), in Manaus. The group participated in iGEM 2014 and won a gold medal in its category by creating a strain of genetically modified bacteria that can detect, absorb, and break down mercury compounds found in water. The idea is to use these microorganisms \u2013 which, according to the researchers, could be called genetically modified machines \u2013 to rid the Amazon\u2019s watersheds of this highly toxic heavy metal. The region\u2019s rivers suffer mercury contamination primarily because the metal is utilized in gold mining.<\/p>\n

The same team will now head to Boston to present a new phase in the study: the prototype of a bioreactor for cleaning mercury-contaminated water. The team raised R$42,000 towards its participation through a crowdfunding campaign and also drew support from private enterprise: Google gave R$15,000 and the Brazilian cosmetics firm Natura donated R$13,000. UFAM covered registration costs, while UEA helped its students by providing airfare. \u201cGetting undergraduate students involved in competitions like iGEM has concrete benefits in training new researchers. They learn how to engage in teamwork and strive to put a practical spin on the knowledge they\u2019ve learned in the classroom,\u201d says Carlos Gustavo Nunes da Silva, professor of genetic engineering at UFAM and coordinator of the project, whose members include 10 students and three professors.<\/p>\n","protected":false},"excerpt":{"rendered":"Student club prepares teams to take part in international competition","protected":false},"author":421,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_exactmetrics_skip_tracking":false,"_exactmetrics_sitenote_active":false,"_exactmetrics_sitenote_note":"","_exactmetrics_sitenote_category":0,"footnotes":""},"categories":[166],"tags":[209,259,243],"coauthors":[740],"class_list":["post-235298","post","type-post","status-publish","format-standard","hentry","category-policies-st-en","tag-biology","tag-chemistry","tag-innovation"],"acf":[],"_links":{"self":[{"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/posts\/235298","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/users\/421"}],"replies":[{"embeddable":true,"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/comments?post=235298"}],"version-history":[{"count":0,"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/posts\/235298\/revisions"}],"wp:attachment":[{"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/media?parent=235298"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/categories?post=235298"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/tags?post=235298"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/coauthors?post=235298"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}