{"id":245610,"date":"2017-08-29T14:49:49","date_gmt":"2017-08-29T17:49:49","guid":{"rendered":"http:\/\/revistapesquisa.fapesp.br\/?p=245610\/"},"modified":"2017-08-29T14:49:49","modified_gmt":"2017-08-29T17:49:49","slug":"india-ink-generates-3-d-image-of-blood-vessels","status":"publish","type":"post","link":"https:\/\/revistapesquisa.fapesp.br\/en\/india-ink-generates-3-d-image-of-blood-vessels\/","title":{"rendered":"India ink generates 3-D image of blood vessels"},"content":{"rendered":"<div id=\"attachment_245611\" style=\"max-width: 310px\" class=\"wp-caption alignright\"><a href=\"http:\/\/revistapesquisa.fapesp.br\/wp-content\/uploads\/2017\/08\/012-017_notas_253-4.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-245611\" src=\"http:\/\/revistapesquisa.fapesp.br\/wp-content\/uploads\/2017\/08\/012-017_notas_253-4-300x268.jpg\" alt=\"\" width=\"300\" height=\"268\" \/><p class=\"wp-caption-text\"><span class=\"media-credits-inline\">EPM\/Unifesp  <\/span><\/a> Simple technique determines the number, length and surface area of blood vessels<span class=\"media-credits\">EPM\/Unifesp  <\/span><\/p><\/div>\n<p>Rather than fluorescent proteins or complex dyes, it was India ink mixed with gelatin\u2014products that can be bought in a store. Using these inexpensive, easy-to-find ingredients, researchers from the Federal University of S\u00e3o Paulo (Unifesp) and the University of Surrey, England, have developed a technique to examine and quantify the blood vessels of the brain by generating high-resolution 3-D images<em>.<\/em> Using a confocal microscope, they were able to record details of vascular branching in several regions of the mouse brain, such as the cerebral cortex, the hippocampus and the cerebellum. The animals received injections of the ink-gelatin compound, which fills the vessels and is visible in brain images (<em>Journal of Anatomy<\/em>, December 2016<em>)<\/em>. The method makes it possible to determine the number, length and surface area of vessels. Changes in these parameters may indicate circulatory disorders. \u201cThe technique is simple to use and the materials are quite accessible,\u201d says parasitologist Renato Mortara, a professor at the Paulista School of Medicine at Unifesp and a coauthor of the study. Preparation of the mixture used in the test takes about 24 hours. The next step in the project is to test the use of the procedure in post-mortem analyses and in biopsies of human tissue. The technique was developed by Robson Gutierre, a postdoctoral researcher at Unifesp.<\/p>\n","protected":false},"excerpt":{"rendered":"India ink mixed with gelatin generates 3-D image of blood vessels","protected":false},"author":475,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_exactmetrics_skip_tracking":false,"_exactmetrics_sitenote_active":false,"_exactmetrics_sitenote_note":"","_exactmetrics_sitenote_category":0,"footnotes":""},"categories":[1651],"tags":[247,236],"coauthors":[785],"class_list":["post-245610","post","type-post","status-publish","format-standard","hentry","category-notes","tag-medicine","tag-physiology"],"acf":[],"_links":{"self":[{"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/posts\/245610","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/users\/475"}],"replies":[{"embeddable":true,"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/comments?post=245610"}],"version-history":[{"count":0,"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/posts\/245610\/revisions"}],"wp:attachment":[{"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/media?parent=245610"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/categories?post=245610"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/tags?post=245610"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/coauthors?post=245610"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}