{"id":54847,"date":"2000-09-13T18:37:13","date_gmt":"2000-09-13T21:37:13","guid":{"rendered":"http:\/\/revistapesquisa.fapesp.br\/?p=54847"},"modified":"2013-01-16T13:07:34","modified_gmt":"2013-01-16T15:07:34","slug":"natural-insect-bank","status":"publish","type":"post","link":"https:\/\/revistapesquisa.fapesp.br\/en\/natural-insect-bank\/","title":{"rendered":"Natural insect bank"},"content":{"rendered":"

A collection of test tubes containing samples of fungi, viruses, bacteria, nematodes and protozoa is changing the way pests that destroy Brazilian agriculture is combated. Arriving in a constant stream from all over Brazil, these samples comprise the inventory of the Microorganism Bank that was set up at the Biological Control Laboratory of the Experimental Center of the Biological Institute in Campinas. Based on these samples, natural biological insecticides can be bred which will substitute chemical pesticides in the fight against nation-wide pests such as the migratory locusts, the insect\u00a0 \u201cpercevejo-de-renda\u201d(Leptopharsa heveae<\/em>)\u00a0 which attacks rubber plantations, the beetle \u201cmoleque-da-bananeira\u201d (Cosmopolite sordidus)<\/em> which attacks the banana, and the citrus borer. And the advantage is that they are non-toxic. \u201cThere is no anecdotal evidence of plants, men and animals having been attacked by entopathogenic micro-organisms\u201d, says Jos\u00e9 Eduardo Marcondes de Almeida, the curator of the bank. There is one more reason for celebration: \u201cThe bio-insecticides can be administered in the same way as chemical pesticides, but without any specialized equipment for the purpose.\u201d<\/p>\n

The entopathogenic microorganisms \u2013 which do not affect plants or animals but cause disease in insects \u2013 are kept in cold storage which resemble vaccine production centers, such is the level of sterilization. These micro-organisms form the basis for the raw material which is supplied for the studies in progress for developing biological pest control, taking place in S\u00e3o Paulo, Paran\u00e1, Bahia and Pernambuco (see box). It is also from this material that the\u00a0 Oldemar Cardim Abreu Entopathogenic Micro-organism Bank \u2013 so-called in honor of the research scientist at the Experimental Center in Campinas who, at the end of the seventies, began studies into the production and storage of the Metarhizium anisopliae<\/em> fungus \u2013 produces six natural pesticides. And this is not all: last year, the bank produced 1.5 tons of\u00a0 natural biological pesticides, sold to farmers and agribusiness throughout the country, both in liquid and solid form at a price close to cost (R$ 20 per liter or R$ 15 per kilo).<\/p>\n

Dynamism
\n<\/strong>According to Antonio Batista Filho, who coordinates the bank and directs the Experimental Center, these results are due to major turnabout in the history of the bank. Created in 1982 by an agronomist, Benedicto Pedro Bastos Cruz, its progress can be described as discreet until August last year. This was when, with R$ 44.3 thousand in financial support from FAPESP, the bank began to be reorganized as part of the Formation of a Entopathogenic Micro-organisms Bank in the Biological Control Laboratory under the coordination of the Experimental Center of the Biological Institute<\/em>. The bank was given a major boost with new insect breeding rooms, incubators, microscopes and refrigerators for storing micro-organisms, such that the numbers in the collection rose from 63 to 260 isolated samples.<\/p>\n

Each type of micro-organism gets the treatment it deserves. Fungi samples are stored in test tubes in a culture with sterile mineral oil in a refrigerator or in eppendorfs (small plastic tubes with lids) in the freezer. With the major changes introduced last year, the 63 samples \u2013 previously conserved by an old\u00a0 technique in pre-cooked rice \u2013 were re-isolated and\u00a0 are now stored in the eppendorfs and culture tubes.<\/p>\n

The bacteria are also held in culture tubes in the refrigerator, the viruses held in suspension and dead insects in the freezer. Meanwhile, the nematodes are preserved in cultures and the protozoa, in dead insects in the freezer. As the microorganisms may be needed for new research at any moment, they are maintained alive, albeit in a state of dormancy \u2013 almost hibernation.<\/p>\n

Growth
\n<\/strong>Almeida was 14 and still lived with his parents in Campinas when he decided: from then on, he would seriously study the mechanisms that control agricultural pests, without polluting the environment and contaminating plants, animals and man himself. In other words, an alternative to chemical pesticides that were beginning to be looked upon unfavorably. With this background, it is easy to understand the ill-concealed pride with which today, at 32, he displays the inventory of samples in the Micro-organisms Bank.<\/p>\n

Almeida fulfilled his childhood dream, but as an agronomist, he has still not reached his objective. He says that for the bank to support the demand from all the programs for the biological control of pests, it will be necessary to obtain at least 500 fungi isolations, 25 for viruses, bacteria and for nematodes of the most important species. That is almost double the existing inventory. However, this is not an impossible mission: every week, an average of ten new microorganism samples is received.<\/p>\n

Up to now, at least 80% of the micro-organisms in storage are in the form of fungi. These dominate because they act on insects by ingestion and contact. For this reason, they are easier to handle during collection, identification, isolation and production of the natural pesticides. But it is not always so easy.\u00a0 The development of isolation techniques, conservation, handling of viruses, bacteria, nematodes and protozoa, that provoke infections when ingested by insects, are still at a preliminary stage of development. \u201cIn a dead insect\u201d, Almeida reminds us, \u201cbacteria deteriorate in two or three days, while the deterioration of the viruses takes up to ten days\u201d.<\/p>\n

The team of five research scientists and two technicians traveled throughout the State of S\u00e3o Paulo in search of samples to establish the inventory. They collected the material from different regions because on many occasions, microorganisms of the same species have genetic peculiarities and attack different types of pest. As he speaks of the origins of the inventory, Almeida does not fail to emphasize the exchange of samples among similar banks of the College of Agriculture Luiz de Queiroz (Esalq), of the University of S\u00e3o Paulo (USP) and the S\u00e3o Paulo State University (Unesp) in Jaboticabal.<\/p>\n

Interchanges<\/strong>
\nThe Experimental Center supplies sample material to banks which are being set up \u2013 such as the State University of Londrina, in the state of Paran\u00e1, and the Federal University of Lavras, in the state of Minas Gerais. It also maintains interchange agreements with overseas centers such as the University of Utah, in the USA, the Ministry of Agriculture and Fisheries in Costa Rica, and the Cuban Institute of Vegetable Sanitation.<\/p>\n

Only those public institutions that are researching microbial control of insects or the genetic improvement of micro-organisms, which are beneficial to agriculture, receive sample material. There is one indispensable precaution: a documented signed by researcher responsible for the institution that the material will only be used for research. Supply to companies that actually produce the natural pesticides occurs only after the signing of an agreement for royalty payments.<\/p>\n

Current research is focused on five species. Three are fungi: Metarhizium anisopliae<\/em>, Beauveria bassiana<\/em> and Sporothrix insectorum<\/em>. Additionally, there is a virus, Baculovirus<\/em>. The fifth is a bacterium, Bacilus thuringiensis<\/em>. Each one of them merits a quick description. Metarhizium<\/em> attacks the largest number of insects and pests. Two examples: the citrus borer, which does significant damage to orange and lemon trees, and the migratory locust, which causes grass for animal forage to turn yellow, thus adversely affecting meat and milk production.<\/p>\n

Beauveria<\/em> is another fungus with wide-ranging action: it infects the coffee and banana borer pest, among others. The Sporothrix<\/em> controls the \u201cpercevejo-de-renda\u201d (leptopharsa heveae)<\/em> which is the main pest to attach rubber plantations in the State of S\u00e3o Paulo. The Baculovirus<\/em> is the virus which is central to one of the world\u2019s leading programs for biological control for combating the soybean lizard \u2013 a pest which eats the plant leaf and drastically reduces output. Finally, the Bacilus thuringiensis<\/em> controls various types of lizard, among which, the soybean lizard and the tomato moth.<\/p>\n

Banana Plants<\/strong>
\nThe discovery of more virulent micro-organisms has enabled the formulation and production of specific natural pesticides. This is the case with the CB 66, a strain from the fungus, Beauveria bassiana<\/em>, that attacks the red mites of the Paraguayan tea plant. It also controls the borer, known locally as \u201cmoleque-da-bananeira\u201d (cosmopolite sordidus)<\/em>, that perforates the root and the trunk of the banana plant. The \u201cmoleque-da-banana\u201d further transmits such diseases as the one caused by the Fusarium oxysporum f. sp. cubense<\/em> which also attacks the banana plant and contaminates the soil and is a growing danger.<\/p>\n

\u201cIn S\u00e3o Paulo, the varieties of apple (ma\u00e7a) and silver (prata) banana are nearing extinction due to the <\/strong>Fusarium oxysporum f. sp. cubense<\/em>\u201d, informs Batista Filho. Since 1995, the Biological Center has played a part in the use of the Beauveria<\/em> fungus in the combating of the banana borer. The fungus was originally developed for the banana growing areas of the Ribeira Valley in S\u00e3o Paulo. The use of this natural pesticide has contributed to preserve the ecosystem and assure the quality of life for the inhabitants of the region.<\/p>\n

The Ribeira Valley experience was a testimony to the Bank\u2019s significance and showed the way to new needs. Since last year, the research scientists have been working on the intensive production of bioinsecticides based on live fungi with funding of R$ 50 thousand from FAPESP for the project, \u201cDevelopment of Production Processes and Formulation of Beauveria bassiana, Metarhizium anisopliae and Sporothrix insectorum<\/em>\u201d. The objective is to solve a problem: the problem of conservation which will allow these products to be kept at room temperature where no scheme for refrigeration is available \u2013 a not uncommon situation in rural areas in Brazil. With this in mind, already in 1997, the team from the Biological Center formulated a natural oil-based pesticide with Baculov\u00edrus anticarsia<\/em>, which showed an efficiency that was over 80% for the control of the soybean lizard. And the conservation goal was reached: the product can now be stored in ordinary cupboards for up to two years.<\/p>\n

High Production
\n<\/strong>Once it is certain that a formulation is going to work, production grows rapidly. An example: during the last harvest, the rubber latex producers of the state of S\u00e3o Paulo, the largest domestic producer, sprayed 5 thousand hectares of rubber plantations with 5 thousand liters of liquid natural pesticide, CB 79. Prepared with a strain of the fungus Sporothrix insectorum<\/em>, CB 79 controls one of the most undesirable pests to attack rubber plantations, known locally as \u201cpercevejo-de-renda\u201d (leptopharsa heveae)<\/em> which reduces the plants\u2019 levels of photosynthesis, adversely affecting rubber latex output. In addition, more than 460 kilos of the E9 natural pesticide made from the Metarhizium anisopliae<\/em> fungus has been used to combat the citrus borer and the migratory locust, mainly in the Para\u00edba Valley.<\/p>\n

Natural pesticides for the biological control of pests are also produced by companies, in some cases using technologies transferred by research institutes such as the Biological Center. The sales amount to significant values. Almeida estimates that 20 tons of natural pesticide with Baculov\u00edrus<\/em> is applied on more than a million hectares of soybean plantations and worth more than US$ 2 million annually. The use in Brazil of mixtures containing Bacillus thuringiensis<\/em> \u2013 one of the most important bacterium for controlling several types of lizard and believed to account for 60% of world sales of natural insecticides\u00a0 \u2013\u00a0 is just shy of 160 tons annually and produce revenues of US$ 2.5 million. Every year, agriculture in Brazil consumes something around 66 tons of fungus-based natural insecticides: 55 tons of Metarhizium anisopliae<\/em>, 3 of Beauveria bassiana <\/em>and <\/em>8 of Sporothrix insectorum<\/em>, equivalent to sales of approximately US$ 1 million.<\/p>\n

Genetic Improvement<\/strong>
\nAs soon as possible, the team at the Biological Center plans will begin studies for the genetic characterization and improvement of the micro-organisms. The other objective: to take steps to establish the patents to protect the natural insecticides. Because the evidence is already clear: there is still huge potential to be exploited. According to Almeida, there are more than 1 million species of identified insects in the world that are known to cause at least one kind of disease.<\/p>\n

\u201cIn the twenty-first century, methods of biological control will be one of the main methods of fighting pests\u201d, says the scientist who, while excited by the prospect, remains firmly focused on the reality of the situation. According to him, the environmental impact must be examined in the light of the number of micro-organisms and the area where they are applied. \u201cFor starters\u201d, he says, \u201cwe believe that the environmental impact is very limited or practically zero when compared with the use of chemical pesticides.\u201d<\/p>\n

Profile<\/strong>:
\n\u2022 Ant\u00f4nio Batista Filho, born in the city of S\u00e3o Paulo, 42, graduated as an agronomist in 1981 and concluded his Ph.D. 1997, in Entomology at the College of Agriculture Luiz de Queiroz (Esalq) of the University of S\u00e3o Paulo (USP). He is coordinator of the Oldemar Cardim Abreu Entopathogenic Micro-organisms Bank and director of the Experimental Center of the Biological Institute of the Department for Agriculture and Supply of the state of S\u00e3o Paulo in Campinas, where he is a research scientist.<\/p>\n

\u2022 Jos\u00e9 Eduardo Marcondes de Almeida, born in Campinas, 32. Graduated as an agronomist in 1990 at the Federal University of Lavras, State of Minas Gerais, and concluded his Ph.D. in Entomology two years ago at College of Agriculture Luiz de Queiroz (Esalq) of the University of S\u00e3o Paulo (USP). He is curator of the Micro-organisms Bank and has been doing research work at the Biological Institute since 1998.<\/p>\n

Project<\/strong>: Formation of a Bank of Entopathogenic Microorganisms in the Biological Control Laboratory at the Experimental Center of the Biological Institute.
\n<\/em>Investment<\/strong>: R$ 44.327,50<\/p>\n

Good news from all sides<\/strong>
\n<\/em>There are eight projects in progress based on micro-organism samples sent by the Biological Institute of Campinas. In one of the laboratories of the Institute itself in Pindamonhangaba, research scientist and agronomist, H\u00e9lio Minoru Takada is testing the control of the rice water weevil, Oryzophagus oryzae<\/em>, with the Beauveria bassiana<\/em> CB 74 and has proved that it causes a 70% mortality rate in the population of the pest.<\/p>\n

At the State University of Campinas (Unicamp), another research scientist at the Biological Center, Leila Barci, a biomedical specialist, selects micro-organisms from two fungi, the Beauveria bassiana<\/em> and the Metarhizium anisopliae<\/em>, for the control of cattle ticks. This is the theme of her Ph.D. thesis.<\/p>\n

At the University of West Paran\u00e1, in Cascavel, the biologist Renato Cassol, under the guidance of Professor Luiz Francisco Alves, studies the control of the striped mite which attacks the mate tea plant. As a rule, chemical pesticides cannot be used in the cultivation of this plant since the use of such would contaminate the leaves which are consumed directly.<\/p>\n

Almeida himself is coordinating a project for the control the cicada which attacks the roots of sugar cane causing the yellowing of the crop in the state of S\u00e3o Paulo and losses of up to 30% in tons per hectare of sugar cane. The agronomist, Lu\u00eds Garrig\u00f3s Leite, of the Experimental Center of the Biological Institute, is preparing his Ph.D. at the University of Utah into the development of methods to produce three species of fungi pertaining to the Entomophthorales<\/em> group. He intends to use these in the combat of cicadas and mites. Leite has already discovered the effect of salts, vitamins, amino acids and sugars in the growth of these species.<\/p>\n","protected":false},"excerpt":{"rendered":"Collection of fungi, viruses and bacteria help combat agricultural pests ","protected":false},"author":6,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_exactmetrics_skip_tracking":false,"_exactmetrics_sitenote_active":false,"_exactmetrics_sitenote_note":"","_exactmetrics_sitenote_category":0,"footnotes":""},"categories":[159],"tags":[],"coauthors":[93],"class_list":["post-54847","post","type-post","status-publish","format-standard","hentry","category-science"],"acf":[],"_links":{"self":[{"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/posts\/54847","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/comments?post=54847"}],"version-history":[{"count":0,"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/posts\/54847\/revisions"}],"wp:attachment":[{"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/media?parent=54847"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/categories?post=54847"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/tags?post=54847"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/revistapesquisa.fapesp.br\/en\/wp-json\/wp\/v2\/coauthors?post=54847"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}